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KMID : 1123920130270060738
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Korean Journal of Oriental Physiology and Pathology
2013 Volume.27 No. 6 p.738 ~ p.744
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Effect of White Ginseng-Ejung-tang Water Extract on Cytokine Production in LPS-induced RAW 264.7 Mouse Macrophages
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Park Wan-Su
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Abstract
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The purpose of this study is to investigate effects of White Ginseng-Ejung-tang water extract (EJ) on production of various cytokines such as interleukin (IL)-2, IL-5, IL-6, IL-10, IL-12p70, macrophage inflammatory protein (MIP)-2, vascular endothelial growth factor (VEGF), keratinocyte-derived chemokine(KC), tumor necrosis factor (TNF)-α, and granulocyte macrophage colony-stimulating factor (GM-CSF) in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. EJ significantly decreased levels of IL-2, IL-12p70, IL-5, MIP-2 for 24 h incubation at the concentrations of 25, 50, and 100 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). EJ significantly decreased levels of IL-6 at the concentrations of 50 and 100 ㎍/m (P < 0.05). EJ significantly decreased levels of IL-10 and VEGF at the concentrations of 25 and 100 ㎍/mL (P < 0.05). EJ significantly decreased levels of KC at the concentrations of 100 ㎍/mL (P < 0.05). EJ did not show any significant effect on TNF-α and GM-CSF production. These results suggest that EJ has anti-inflammtory property related with its inhibition of IL-2, IL-5, IL-6, IL-10, IL-12p70, MIP-2, VEGF, and KC production in LPS-induced macrophages.
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KEYWORD
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White Ginseng, Ejung-tang, Macrophage, Inflammation, Cytokine, Interleukin
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